4H2F

Human ecto-5'-nucleotidase (CD73): crystal form I (open) in complex with adenosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	5'-nucleotidase	polymer	546	60446.4	1	UniProt (P21589) Pfam (PF00149) Pfam (PF02872)	Homo sapiens (human)	5'-NT, Ecto-5'-nucleotidase
2	B (A)	ADENOSINE	non-polymer		267.2	1	Chemie (ADN)
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	E (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	F (A)	water	water		18.0	504	Chemie (HOH)

Sequence modifications

A: 27 - 549 (UniProt: P21589)

PDB	External Database	Details
Met 4	-	expression tag
Ala 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Val 12	-	expression tag
Gly 13	-	expression tag
Thr 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Asn 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Asp 20	-	expression tag
Asp 21	-	expression tag
Lys 22	-	expression tag
Ser 23	-	expression tag
Pro 24	-	expression tag
Asp 25	-	expression tag
Pro 26	-	expression tag
Asp 53	Asn 53	engineered mutation
Asp 311	Asn 311	engineered mutation
Asp 333	Asn 333	engineered mutation
Ala 376	Thr 376	variant
Asp 403	Asn 403	engineered mutation
Ala 478	Lys 478	engineered mutation
Ala 480	Arg 480	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	60446.4
Non-Polymers*	Number of molecules	4
	Total formula weight	408.2
All*	Total formula weight	60854.6

*Water molecules are not included.