4H2F

Human ecto-5'-nucleotidase (CD73): crystal form I (open) in complex with adenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-09
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 43 3 2
Unit cell lengths	167.548, 167.548, 167.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 1.850
R-factor	0.15754
Rwork	0.157
R-free	0.17929
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.378
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.890
High resolution limit [Å]	1.850
Number of reflections	68775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	292	1.0 M lithium chloride, 0.1 M Hepes , pH 7.0, VAPOR DIFFUSION, temperature 292K