4GZD

Crystal structure of yeast Ent2 ENTH domain triple mutant N112D,S114E, E118Q

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Epsin-2	polymer	180	21064.8	1	UniProt (Q05785) Pfam (PF01417)	Saccharomyces cerevisiae (Baker's yeast)
2	B (A)	ISOPROPYL ALCOHOL	non-polymer		60.1	1	Chemie (IPA)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D (A)	water	water		18.0	81	Chemie (HOH)

Sequence modifications

A: 1 - 149 (UniProt: Q05785)

PDB	External Database	Details
Met -30	-	expression tag
Gly -29	-	expression tag
Ser -28	-	expression tag
Ser -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Leu -17	-	expression tag
Val -16	-	expression tag
Pro -15	-	expression tag
Arg -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
Met -10	-	expression tag
Ala -9	-	expression tag
Met -8	-	expression tag
Glu -7	-	expression tag
Ala -6	-	expression tag
Glu -5	-	expression tag
Phe -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ile -1	-	expression tag
Gln 0	-	expression tag
Tyr 5	Phe 5	conflict
Asp 112	Asn 112	engineered mutation
Glu 114	Ser 114	engineered mutation
Gln 118	Glu 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	21064.8
Non-Polymers*	Number of molecules	2
	Total formula weight	95.5
All*	Total formula weight	21160.3

*Water molecules are not included.