4GSL

Crystal structure of an Atg7-Atg3 crosslinked complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ubiquitin-like modifier-activating enzyme ATG7	polymer	615	69692.2	2	UniProt (P38862) Pfam (PF16420) Pfam (PF00899)	Saccharomyces cerevisiae (yeast)	ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Cytoplasm to vacuole targeting protein 2
2	C, D (C, D)	Autophagy-related protein 3	polymer	312	35975.0	2	UniProt (P40344) Pfam (PF03987)	Saccharomyces cerevisiae (yeast)	Autophagy-related E2-like conjugation enzyme ATG3
3	E, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	G, H, I (A, B, C)	water	water		18.0	43	Chemie (HOH)

Sequence modifications

A, B: 1 - 613 (UniProt: P38862)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

C, D: 1 - 310 (UniProt: P40344)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 41	Cys 41	engineered mutation
Ala 76	Cys 76	engineered mutation
Ala 83	Cys 83	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	211334.4
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	211465.2

*Water molecules are not included.