4GSK

Crystal structure of an Atg7-Atg10 crosslinked complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ubiquitin-like modifier-activating enzyme ATG7	polymer	615	69628.0	2	UniProt (P38862) Pfam (PF16420) Pfam (PF00899) In PDB	Saccharomyces cerevisiae (yeast)	ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Cytoplasm to vacuole targeting protein 2
2	Y, Z	Ubiquitin-like-conjugating enzyme ATG10	polymer	173	20147.8	2	UniProt (Q07879) Pfam (PF03987) In PDB	Saccharomyces cerevisiae (yeast)	Autophagy-related protein 10
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 1 - 613 (UniProt: P38862)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 39	Cys 39	engineered mutation
Ser 195	Cys 195	engineered mutation
Ala 375	Cys 375	engineered mutation

Y, Z: 1 - 167 (UniProt: Q07879)

PDB	External Database	Details
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ser 26	Cys 26	engineered mutation
Ser 137	Cys 137	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	179551.6
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	179682.5

*Water molecules are not included.