4GJS

Streptavidin-K121H

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Streptavidin	polymer	159	16579.0	2	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	A, B	trichloro{(1,2,3,4,5-eta)-1,2,3,4-tetramethyl-5-[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]cyclopentadienyl}rhodium(1+)	non-polymer		599.8	2	Chemie (0OD)
3	A, B	Rhodium	non-polymer		102.9	2	Chemie (RH)
4		water	water		18.0	327	Chemie (HOH)

Sequence modifications

A, B: 2 - 159 (UniProt: P22629)

PDB	External Database	Details
Met 1	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
His 121	Lys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	33158.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1405.5
All*	Total formula weight	34563.4

*Water molecules are not included.