4GJS
Streptavidin-K121H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.652, 81.331, 47.002 |
| Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
| Resolution | 28.321 - 1.850 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALA (CCP4_3.3.20 2011/05/18) |
| Refinement software | PHENIX (dev_1050) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.670 | 40.670 | 1.950 |
| High resolution limit [Å] | 1.850 | 5.850 | 1.850 |
| Rmerge | 0.081 | 0.100 | 0.180 |
| Total number of observations | 2216 | 7608 | |
| Number of reflections | 24972 | ||
| <I/σ(I)> | 5.9541 | 5.55 | 3.55 |
| Completeness [%] | 98.5 | 94.75 | 96.35 |
| Redundancy | 2.65 | 2.84 | 2.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M MES, 19 % PEG500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
