4GIZ

Crystal structure of full-length human papillomavirus oncoprotein E6 in complex with LXXLL peptide of ubiquitin ligase E6AP at 2.55 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Maltose-binding periplasmic protein, UBIQUITIN LIGASE EA6P: chimeric protein	polymer	382	41672.1	2	UniProt (P0AEX9) UniProt (Q05086) Pfam (PF01547) In PDB	Escherichia coli	MBP, MMBP, Maltodextrin-binding protein
2	C, D	Protein E6	polymer	142	17135.7	2	UniProt (P03126) Pfam (PF00518) In PDB	Human papillomavirus type 16
3	E, F	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		990.9	2	In PDB BIRD (PRD_900035) GlyTouCan (G91368RN)		alpha-maltohexaose
4	C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5		water	water		18.0	572	Chemie (HOH)

Sequence modifications

A, B: 2 - 367 (UniProt: P0AEX9)

PDB	External Database	Details
Ala 83	Asp 108	engineered mutation
Ala 84	Lys 109	engineered mutation
Ala 240	Lys 265	engineered mutation
Ala 360	Glu 385	engineered mutation
Ala 363	Lys 388	engineered mutation
Ala 364	Asp 389	engineered mutation

A, B: 372 - 383 (UniProt: Q05086)
C, D: 2 - 143 (UniProt: P03126)

PDB	External Database	Details
Arg 47	Phe 54	engineered mutation
Ser 80	Cys 87	engineered mutation
Ser 97	Cys 104	engineered mutation
Ser 111	Cys 118	engineered mutation
Ser 140	Cys 147	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117615.6
Branched	Number of molecules	2
	Total formula weight	1981.7
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	119858.9

*Water molecules are not included.