4GIZ
Crystal structure of full-length human papillomavirus oncoprotein E6 in complex with LXXLL peptide of ubiquitin ligase E6AP at 2.55 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-05 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.279, 134.936, 138.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.330 - 2.550 |
R-factor | 0.1677 |
Rwork | 0.166 |
R-free | 0.19570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.330 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.082 | 0.537 |
Number of reflections | 65602 | |
<I/σ(I)> | 31 | 2.9 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 7.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 290 | 10% peg8000, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |