4GIS

crystal structure of an enolase family member from vibrio harveyi (efi-target 501692) with homology to mannonate dehydratase, with mg, glycerol and dicarboxylates bound (mixed loops, space group I4122)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Enolase	polymer	422	48225.2	2	UniProt (D0X4R4) Pfam (PF02746) Pfam (PF13378)	Vibrio harveyi
2	C, H, I, J (A, B)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
3	D, F, K (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	E (A)	MALONIC ACID	non-polymer		104.1	1	Chemie (MLA)
5	G, L (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	M (B)	MALEIC ACID	non-polymer		116.1	1	Chemie (MAE)
7	N, O (A, B)	water	water		18.0	788	Chemie (HOH)

Sequence modifications

A, B: 1 - 399 (UniProt: D0X4R4)

PDB	External Database	Details
Met -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	96450.4
Non-Polymers*	Number of molecules	11
	Total formula weight	686.8
All*	Total formula weight	97137.2

*Water molecules are not included.