4GHF
Structure of Y257F variant of Homoprotocatechuate 2,3-Dioxygenase from B.fuscum in complex with 4-Nitrocatechol and dioxygen at 1.67 Ang resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Homoprotocatechuate 2,3-dioxygenase | polymer | 365 | 41739.3 | 4 | UniProt (Q45135) Pfam (PF00903) | Brevibacterium fuscum | |
| 2 | E, I, O, U (A, B, C, D) | FE (II) ION | non-polymer | 55.8 | 4 | Chemie (FE2) | |||
| 3 | F, P, V (A, C, D) | 4-NITROCATECHOL | non-polymer | 155.1 | 3 | Chemie (4NC) | |||
| 4 | G, M, N, R, W (A, B, C, D) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 5 | Chemie (P6G) | |||
| 5 | H, J, T, Y (A, B, C, D) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 6 | K (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 7 | L, Q (B, C) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
| 8 | S, X (C, D) | OXYGEN MOLECULE | non-polymer | 32.0 | 2 | Chemie (OXY) | |||
| 9 | AA, BA, CA, Z (B, C, D, A) | water | water | 18.0 | 1169 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 365 (UniProt: Q45135)
| PDB | External Database | Details |
|---|---|---|
| Phe 257 | Tyr 257 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 166957.3 | |
| Non-Polymers* | Number of molecules | 21 |
| Total formula weight | 2734.7 | |
| All* | Total formula weight | 169692.0 |
