4GH7

Crystal structure of Anticalin N7A in complex with oncofetal fibronectin fragment Fn7B8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Neutrophil gelatinase-associated lipocalin	polymer	188	21993.9	2	UniProt (P80188) Pfam (PF00061)	Homo sapiens (human)	NGAL, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25
2	B, D (B, D)	Fibronectin	polymer	285	30966.1	2	UniProt (P02751) Pfam (PF00041)	Homo sapiens (human)	FN
3	E, F, G, H (A, B, C, D)	water	water		18.0	164	Chemie (HOH)

Sequence modifications

A, C: 1 - 178 (UniProt: P80188)

PDB	External Database	Details
His 28	Gln 48	engineered mutation
Lys 36	Leu 56	engineered mutation
His 40	Ala 60	engineered mutation
Asp 41	Ile 61	engineered mutation
Arg 49	Gln 69	engineered mutation
Gln 52	Tyr 72	engineered mutation
Asn 68	Ser 88	engineered mutation
Arg 70	Leu 90	engineered mutation
Val 72	Arg 92	engineered mutation
His 73	Lys 93	engineered mutation
Asn 77	Asp 97	engineered mutation
Arg 79	Trp 99	engineered mutation
Trp 81	Arg 101	engineered mutation
Ser 87	Cys 107	engineered mutation
Trp 100	Tyr 120	engineered mutation
Trp 106	Tyr 126	engineered mutation
Arg 125	Lys 145	engineered mutation
Tyr 127	Ser 147	engineered mutation
Leu 132	Tyr 152	engineered mutation
Glu 134	Lys 154	engineered mutation
Asn 146	Ser 166	engineered mutation
Ser 179	-	expression tag
Ala 180	-	expression tag
Trp 181	-	expression tag
Ser 182	-	expression tag
His 183	-	expression tag
Pro 184	-	expression tag
Gln 185	-	expression tag
Phe 186	-	expression tag
Glu 187	-	expression tag
Lys 188	-	expression tag

B, D: 1173 - 1448 (UniProt: P02751)

PDB	External Database	Details
Met 1172	-	expression tag
Ser 1449	-	expression tag
Ala 1450	-	expression tag
His 1451	-	expression tag
His 1452	-	expression tag
His 1453	-	expression tag
His 1454	-	expression tag
His 1455	-	expression tag
His 1456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105920.0
All*	Total formula weight	105920.0

*Water molecules are not included.