4GBR

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-2 adrenergic receptor	polymer	309	35092.2	1	UniProt (P07550) Pfam (PF00001)	Homo sapiens (human)	Beta-2 adrenoreceptor, Beta-2 adrenoceptor
2	B (B)	Lysozyme	polymer	163	18339.9	1	UniProt (P00720) Pfam (PF00959)	Enterobacteria phage T4	Endolysin, Lysis protein, Muramidase
3	C (A)	(2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol	non-polymer		298.4	1	Chemie (CAU)

Sequence modifications

A: 29 - 337 (UniProt: P07550)

PDB	External Database	Details
Thr 96	Met 96	engineered mutation
Thr 98	Met 98	engineered mutation
Glu 187	Asn 187	engineered mutation
-	Lys 235	deletion
-	Ser 236	deletion
-	Glu 237	deletion
-	Gly 238	deletion
-	Arg 239	deletion
-	Phe 240	deletion
-	His 241	deletion
-	Val 242	deletion
-	Gln 243	deletion
-	Asn 244	deletion
-	Leu 245	deletion
-	Ser 246	deletion
-	Gln 247	deletion
-	Val 248	deletion
-	Glu 249	deletion
-	Gln 250	deletion
-	Asp 251	deletion
-	Gly 252	deletion
-	Arg 253	deletion
-	Thr 254	deletion
-	Gly 255	deletion
-	His 256	deletion
-	Gly 257	deletion
-	Leu 258	deletion
-	Arg 259	deletion
-	Arg 260	deletion
-	Ser 261	deletion
-	Ser 262	deletion

B: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1001	-	expression tag
Thr 1054	Cys 54	conflict
Ala 1097	Cys 97	conflict
Ala 1162	-	expression tag
Ala 1163	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53432.1
Non-Polymers*	Number of molecules	1
	Total formula weight	298.4
All*	Total formula weight	53730.5

*Water molecules are not included.