4GBR

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-2 adrenergic receptor	polymer	309	35092.2	1	UniProt (P07550) Pfam (PF00001) In PDB	Homo sapiens (human)	Beta-2 adrenoreceptor, Beta-2 adrenoceptor
2	B	Lysozyme	polymer	163	18339.9	1	UniProt (P00720) Pfam (PF00959) In PDB	Enterobacteria phage T4	Endolysin, Lysis protein, Muramidase
3	A	(2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol	non-polymer		298.4	1	Chemie (CAU)

Sequence modifications

A: 29 - 337 (UniProt: P07550)

PDB	External Database	Details
Thr 96	Met 96	ENGINEERED MUTATION
Thr 98	Met 98	ENGINEERED MUTATION
Glu 187	Asn 187	ENGINEERED MUTATION
-	Lys 235	DELETION
-	Ser 236	DELETION
-	Glu 237	DELETION
-	Gly 238	DELETION
-	Arg 239	DELETION
-	Phe 240	DELETION
-	His 241	DELETION
-	Val 242	DELETION
-	Gln 243	DELETION
-	Asn 244	DELETION
-	Leu 245	DELETION
-	Ser 246	DELETION
-	Gln 247	DELETION
-	Val 248	DELETION
-	Glu 249	DELETION
-	Gln 250	DELETION
-	Asp 251	DELETION
-	Gly 252	DELETION
-	Arg 253	DELETION
-	Thr 254	DELETION
-	Gly 255	DELETION
-	His 256	DELETION
-	Gly 257	DELETION
-	Leu 258	DELETION
-	Arg 259	DELETION
-	Arg 260	DELETION
-	Ser 261	DELETION
-	Ser 262	DELETION

B: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1001	-	EXPRESSION TAG
Thr 1054	Cys 54	CONFLICT
Ala 1097	Cys 97	CONFLICT
Ala 1162	-	EXPRESSION TAG
Ala 1163	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53432.1
Non-Polymers*	Number of molecules	1
	Total formula weight	298.4
All*	Total formula weight	53730.5

*Water molecules are not included.