4GBR

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 130 mm
Spacegroup name	P 21 21 21
Unit cell lengths	51.382, 71.376, 161.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.738 - 3.993
R-factor	0.2677
Rwork	0.265
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.693
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.993
Number of reflections	4927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	PEG300 37% Bis-Tris propane 0.1M Ammonium phosphate, dibasic, 0.1M, pH 6.5, lipidic cubic phase, temperature 293K