4GAD

Crystal Structure of D230A/H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	5'/3'-nucleotidase SurE	polymer	267	28504.0	2	UniProt (P66881) Pfam (PF01975)	Salmonella typhimurium	Exopolyphosphatase, Nucleoside monophosphate phosphohydrolase
2	C, E (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H (A, B)	water	water		18.0	230	Chemie (HOH)

Sequence modifications

A, B: 1 - 253 (UniProt: P66881)

PDB	External Database	Details
Met -13	-	expression tag
Arg -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 230	Asp 230	engineered mutation
Ala 234	His 234	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57008.1
Non-Polymers*	Number of molecules	4
	Total formula weight	232.8
All*	Total formula weight	57240.9

*Water molecules are not included.