4GAD
Crystal Structure of D230A/H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-07-12 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 80.600, 97.330, 127.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.350 |
R-factor | 0.22282 |
Rwork | 0.220 |
R-free | 0.26888 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g9o |
RMSD bond length | 0.006 |
RMSD bond angle | 1.137 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.780 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.073 | 0.482 |
Number of reflections | 21227 | |
<I/σ(I)> | 3.3 | |
Redundancy | 5.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Under Oil, Microbatch Method | 7.5 | 293 | 0.1M HEPES pH 7.5, 0.02M MgCl2 hexahydrate, 30% (w/v) D-glucose, 22% (w/v) polyacrylic acid, Under Oil, Microbatch Method, temperature 293K |