4G7Y

Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (R217E, 2.8 A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	Fragment antibody heavy chain	polymer	219	23092.6	1		Homo sapiens
2	B (L)	Fragment antibody light chain	polymer	211	23103.7	1		Homo sapiens
3	C (S)	Voltage-sensor containing phosphatase	polymer	185	21168.7	1	UniProt (Q4W8A1) Pfam (PF00520)	Ciona intestinalis (sea vase yellow sea squirt)
4	D (L)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	E (L)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	F (S)	LAURYL DIMETHYLAMINE-N-OXIDE	non-polymer		229.4	1	Chemie (LDA)
7	G, H, I (H, L, S)	water	water		18.0	35	Chemie (HOH)

Sequence modifications

S: 89 - 260 (UniProt: Q4W8A1)

PDB	External Database	Details
Met 79	-	EXPRESSION TAG
Arg 80	-	EXPRESSION TAG
Gly 81	-	EXPRESSION TAG
Ser 82	-	EXPRESSION TAG
His 83	-	EXPRESSION TAG
His 84	-	EXPRESSION TAG
His 85	-	EXPRESSION TAG
His 86	-	EXPRESSION TAG
His 87	-	EXPRESSION TAG
His 88	-	EXPRESSION TAG
Glu 217	Arg 217	ENGINEERED MUTATION
Lys 261	-	EXPRESSION TAG
Leu 262	-	EXPRESSION TAG
Asn 263	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	67365.0
Non-Polymers*	Number of molecules	3
	Total formula weight	289.2
All*	Total formula weight	67654.1

*Water molecules are not included.