4G56

Crystal Structure of full length PRMT5/MEP50 complexes from Xenopus laevis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Hsl7 protein	polymer	657	75713.5	2	UniProt (Q6NUA1) Pfam (PF17285) Pfam (PF05185) Pfam (PF17286)	Xenopus laevis (clawed frog common platanna platanna)	Methyltransferase Hsl7
2	B, D (B, D)	MGC81050 protein	polymer	357	38872.7	2	UniProt (Q6NUD0) Pfam (PF00400)	Xenopus laevis (clawed frog common platanna platanna)
3	E, F (A, C)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
4	G, H (A, C)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, C: 2 - 633 (UniProt: Q6NUA1)

PDB	External Database	Details
Mse -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

B, D: 2 - 333 (UniProt: Q6NUD0)

PDB	External Database	Details
Mse -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	229172.4
Non-Polymers*	Number of molecules	2
	Total formula weight	768.8
All*	Total formula weight	229941.2

*Water molecules are not included.