4G56

Crystal Structure of full length PRMT5/MEP50 complexes from Xenopus laevis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Hsl7 protein	polymer	657	75713.5	2	UniProt (Q6NUA1) Pfam (PF17285) Pfam (PF05185) Pfam (PF17286) In PDB	Xenopus laevis (clawed frog common platanna platanna)	Methyltransferase Hsl7
2	B, D	MGC81050 protein	polymer	357	38872.7	2	UniProt (Q6NUD0) Pfam (PF00400) In PDB	Xenopus laevis (clawed frog common platanna platanna)
3	A, C	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
4		water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, C: 2 - 633 (UniProt: Q6NUA1)

PDB	External Database	Details
Mse -23	-	EXPRESSION TAG
His -22	-	EXPRESSION TAG
His -21	-	EXPRESSION TAG
His -20	-	EXPRESSION TAG
His -19	-	EXPRESSION TAG
His -18	-	EXPRESSION TAG
His -17	-	EXPRESSION TAG
Ser -16	-	EXPRESSION TAG
Ser -15	-	EXPRESSION TAG
Gly -14	-	EXPRESSION TAG
Val -13	-	EXPRESSION TAG
Asp -12	-	EXPRESSION TAG
Leu -11	-	EXPRESSION TAG
Gly -10	-	EXPRESSION TAG
Thr -9	-	EXPRESSION TAG
Glu -8	-	EXPRESSION TAG
Asn -7	-	EXPRESSION TAG
Leu -6	-	EXPRESSION TAG
Tyr -5	-	EXPRESSION TAG
Phe -4	-	EXPRESSION TAG
Gln -3	-	EXPRESSION TAG
Ser -2	-	EXPRESSION TAG
Asn -1	-	EXPRESSION TAG
Gly 0	-	EXPRESSION TAG
Ser 1	-	EXPRESSION TAG

B, D: 2 - 333 (UniProt: Q6NUD0)

PDB	External Database	Details
Mse -23	-	EXPRESSION TAG
His -22	-	EXPRESSION TAG
His -21	-	EXPRESSION TAG
His -20	-	EXPRESSION TAG
His -19	-	EXPRESSION TAG
His -18	-	EXPRESSION TAG
His -17	-	EXPRESSION TAG
Ser -16	-	EXPRESSION TAG
Ser -15	-	EXPRESSION TAG
Gly -14	-	EXPRESSION TAG
Val -13	-	EXPRESSION TAG
Asp -12	-	EXPRESSION TAG
Leu -11	-	EXPRESSION TAG
Gly -10	-	EXPRESSION TAG
Thr -9	-	EXPRESSION TAG
Glu -8	-	EXPRESSION TAG
Asn -7	-	EXPRESSION TAG
Leu -6	-	EXPRESSION TAG
Tyr -5	-	EXPRESSION TAG
Phe -4	-	EXPRESSION TAG
Gln -3	-	EXPRESSION TAG
Ser -2	-	EXPRESSION TAG
Asn -1	-	EXPRESSION TAG
Gly 0	-	EXPRESSION TAG
Ser 1	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	229172.4
Non-Polymers*	Number of molecules	2
	Total formula weight	768.8
All*	Total formula weight	229941.2

*Water molecules are not included.