4G2K

Crystal structure of the Marburg Virus GP2 ectodomain in its post-fusion conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	General control protein GCN4, Envelope glycoprotein GP2 chimera	polymer	125	14691.9	3	UniProt (P03069) UniProt (P35254) Pfam (PF07716) Pfam (PF22307)	Saccharomyces cerevisiae (yeast Marburg virus)	Amino acid biosynthesis regulatory protein, virion spike glycoprotein
2	D, F (A, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	E (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	G, H, I (A, B, C)	water	water		18.0	251	Chemie (HOH)

Sequence modifications

A, B, C: 524 - 553 (UniProt: P03069)

PDB	External Database	Details
Met 509	-	expression tag
His 510	-	expression tag
His 511	-	expression tag
His 512	-	expression tag
His 513	-	expression tag
His 514	-	expression tag
His 515	-	expression tag
Glu 516	-	expression tag
Asn 517	-	expression tag
Leu 518	-	expression tag
Tyr 519	-	expression tag
Phe 520	-	expression tag
Gln 521	-	expression tag
Gly 522	-	expression tag
Asn 523	-	expression tag
Ile 527	Leu 253	engineered mutation
Ile 531	Val 257	engineered mutation
Ile 534	Leu 260	engineered mutation
Ile 538	Asn 264	engineered mutation
Ile 541	Leu 267	engineered mutation
Ile 545	Val 271	engineered mutation
Ile 548	Leu 274	engineered mutation
Ile 552	Val 278	engineered mutation

A, B, C: 554 - 633 (UniProt: P35254)

PDB	External Database	Details
Ser 557	Cys 557	engineered mutation
Ser 610	Cys 610	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	44075.7
Non-Polymers*	Number of molecules	3
	Total formula weight	219.6
All*	Total formula weight	44295.3

*Water molecules are not included.