4FYD

Crystal structure of yeast DNA polymerase alpha bound to DNA/RNA and dGTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA polymerase alpha catalytic subunit A	polymer	910	103401.7	2	UniProt (P13382) Pfam (PF03104) Pfam (PF00136) In PDB	Saccharomyces cerevisiae (Baker's yeast)
2	C, D	DNA (5'-D(*TP*GP*AP*GP*CP*GP*TP*G*TP*GP*TP*AP*CP*CP*CP*CP*TP*GP*CP*CP*CP*GP*CP*CP*G)-3')	polymer	25	7627.9	2
3	E, F	DNA/RNA (5'-R(*CP*GP*GP*CP*GP*GP*GP*CP*AP*G)-D(P*GP*G)-3')	polymer	12	3929.4	2
4	A, B	2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (DGT)

Sequence modifications

A, B: 349 - 1258 (UniProt: P13382)

PDB	External Database	Details
Ala 508	Arg 508	engineered mutation
Ala 509	Asn 509	engineered mutation
Asn 998	Asp 998	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	229918.1
Non-Polymers*	Number of molecules	2
	Total formula weight	1014.4
All*	Total formula weight	230932.4

*Water molecules are not included.