4FYD
Crystal structure of yeast DNA polymerase alpha bound to DNA/RNA and dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.689, 145.686, 197.199 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.801 - 3.100 |
R-factor | 0.2116 |
Rwork | 0.210 |
R-free | 0.24670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fvm |
RMSD bond length | 0.003 |
RMSD bond angle | 0.652 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1078)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.390 | 3.260 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.105 | 0.974 |
Number of reflections | 59154 | |
<I/σ(I)> | 10.8 | 2.1 |
Completeness [%] | 99.7 | 98.4 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 291 | 0.2M MgAc2, 10% PEG8000, microbatch, temperature 291K |