4FLY
Pyrococcus abyssi B family DNA polymerase bound to a dsDNA, in edition mode
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | Template strand | polymer | 13 | 4008.6 | 1 | synthetic construct | ||
| 2 | B (P) | Primer strand | polymer | 8 | 2411.6 | 1 | synthetic construct | ||
| 3 | C (A) | DNA polymerase 1 | polymer | 793 | 91809.9 | 1 | UniProt (P0CL77) Pfam (PF03104) Pfam (PF00136) | Pyrococcus abyssi | Pab polymerase |
| 4 | D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 6 | G, H, I (T, P, A) | water | water | 18.0 | 563 | Chemie (HOH) |
Sequence modifications
A: 1 - 771 (UniProt: P0CL77)
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Val -7 | - | expression tag |
| Pro -6 | - | expression tag |
| Ala -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Ser -3 | - | expression tag |
| His -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ala 215 | Asp 215 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 98230.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 243.8 | |
| All* | Total formula weight | 98474.0 |
