4FLW

Pyrococcus abyssi B family DNA polymerase bound to a dsDNA, in edition mode

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase 1	polymer	793	91809.9	1	UniProt (P0CL77) Pfam (PF03104) Pfam (PF00136) In PDB	Pyrococcus abyssi	Pab polymerase
2	P	Primer strand	polymer	11	3399.2	1		synthetic construct
3	T	Template strand	polymer	13	4022.6	1		synthetic construct
4	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7		water	water		18.0	647	Chemie (HOH)

Sequence modifications

A: 1 - 771 (UniProt: P0CL77)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Ala -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Ala -1	-	expression tag
Gly 0	-	expression tag
Ala 215	Asp 215	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	99231.8
Non-Polymers*	Number of molecules	4
	Total formula weight	335.9
All*	Total formula weight	99567.7

*Water molecules are not included.