4FI3
Structure of vitamin B12 transporter BtuCD-F in a nucleotide-bound state
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Vitamin B12 import system permease protein BtuC | polymer | 349 | 37650.6 | 2 | UniProt (P06609) Pfam (PF01032) In PDB | Escherichia coli | |
2 | C, D | Vitamin B12 import ATP-binding protein BtuD | polymer | 249 | 27086.2 | 2 | UniProt (P06611) Pfam (PF00005) In PDB | Escherichia coli | Vitamin B12-transporting ATPase |
3 | F | Vitamin B12-binding protein | polymer | 255 | 28238.1 | 1 | UniProt (P37028) Pfam (PF01497) In PDB | Escherichia coli | |
4 | C, D | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
5 | C, D | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A, B: 1 - 326 (UniProt: P06609)
C, D: 1 - 249 (UniProt: P06611)
F: 22 - 266 (UniProt: P37028)
PDB | External Database | Details |
---|---|---|
Met -22 | - | expression tag |
Gly -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Ser -10 | - | expression tag |
Ser -9 | - | expression tag |
Gly -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
His 0 | - | expression tag |
Ser 18 | Cys 18 | engineered mutation |
Ser 32 | Cys 32 | engineered mutation |
Ser 120 | Cys 120 | engineered mutation |
Ser 156 | Cys 156 | engineered mutation |
Ser 205 | Cys 205 | engineered mutation |
Ser 206 | Cys 206 | engineered mutation |
Ser 267 | Cys 267 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gln 159 | Glu 159 | engineered mutation |
Cys 162 | Asn 162 | engineered mutation |
Ser 180 | Cys 180 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 21 | - | expression tag |
Ser 267 | - | expression tag |
Gly 268 | - | expression tag |
Ser 269 | - | expression tag |
His 270 | - | expression tag |
His 271 | - | expression tag |
His 272 | - | expression tag |
His 273 | - | expression tag |
His 274 | - | expression tag |
His 275 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 157711.7 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 1061.0 | |
All* | Total formula weight | 158772.7 |