4FI3
Structure of vitamin B12 transporter BtuCD-F in a nucleotide-bound state
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0015420 | molecular_function | ABC-type vitamin B12 transporter activity |
| A | 0015889 | biological_process | cobalamin transport |
| A | 0016020 | cellular_component | membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0035461 | biological_process | vitamin transmembrane transport |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0090482 | molecular_function | vitamin transmembrane transporter activity |
| A | 1990191 | cellular_component | cobalamin transport complex |
| A | 1990193 | cellular_component | BtuCD complex |
| B | 0005515 | molecular_function | protein binding |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0015420 | molecular_function | ABC-type vitamin B12 transporter activity |
| B | 0015889 | biological_process | cobalamin transport |
| B | 0016020 | cellular_component | membrane |
| B | 0022857 | molecular_function | transmembrane transporter activity |
| B | 0035461 | biological_process | vitamin transmembrane transport |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| B | 0055085 | biological_process | transmembrane transport |
| B | 0090482 | molecular_function | vitamin transmembrane transporter activity |
| B | 1990191 | cellular_component | cobalamin transport complex |
| B | 1990193 | cellular_component | BtuCD complex |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0015420 | molecular_function | ABC-type vitamin B12 transporter activity |
| C | 0015889 | biological_process | cobalamin transport |
| C | 0016020 | cellular_component | membrane |
| C | 0016887 | molecular_function | ATP hydrolysis activity |
| C | 0019898 | cellular_component | extrinsic component of membrane |
| C | 0042626 | molecular_function | ATPase-coupled transmembrane transporter activity |
| C | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| C | 0055085 | biological_process | transmembrane transport |
| C | 1990191 | cellular_component | cobalamin transport complex |
| C | 1990193 | cellular_component | BtuCD complex |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0015420 | molecular_function | ABC-type vitamin B12 transporter activity |
| D | 0015889 | biological_process | cobalamin transport |
| D | 0016020 | cellular_component | membrane |
| D | 0016887 | molecular_function | ATP hydrolysis activity |
| D | 0019898 | cellular_component | extrinsic component of membrane |
| D | 0042626 | molecular_function | ATPase-coupled transmembrane transporter activity |
| D | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| D | 0055085 | biological_process | transmembrane transport |
| D | 1990191 | cellular_component | cobalamin transport complex |
| D | 1990193 | cellular_component | BtuCD complex |
| F | 0015889 | biological_process | cobalamin transport |
| F | 0031419 | molecular_function | cobalamin binding |
| F | 0042597 | cellular_component | periplasmic space |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE ANP C 301 |
| Chain | Residue |
| C | ARG15 |
| C | GLN159 |
| C | HIS191 |
| C | MG302 |
| D | ARG122 |
| D | GLN126 |
| D | LEU127 |
| D | SER128 |
| D | GLY129 |
| D | GLY130 |
| D | GLU131 |
| C | PRO34 |
| C | ASN35 |
| C | GLY36 |
| C | ALA37 |
| C | GLY38 |
| C | LYS39 |
| C | SER40 |
| C | THR41 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG C 302 |
| Chain | Residue |
| C | SER40 |
| C | GLN80 |
| C | GLN159 |
| C | ANP301 |
| site_id | AC3 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE ANP D 301 |
| Chain | Residue |
| C | ARG122 |
| C | GLN126 |
| C | SER128 |
| C | GLY129 |
| C | GLY130 |
| C | GLU131 |
| D | ARG15 |
| D | PRO34 |
| D | ASN35 |
| D | GLY36 |
| D | ALA37 |
| D | GLY38 |
| D | LYS39 |
| D | SER40 |
| D | THR41 |
| D | GLN159 |
| D | HIS191 |
| D | MG302 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG D 302 |
| Chain | Residue |
| D | SER40 |
| D | GLN80 |
| D | GLN159 |
| D | ANP301 |
Functional Information from PROSITE/UniProt
| site_id | PS00211 |
| Number of Residues | 15 |
| Details | ABC_TRANSPORTER_1 ABC transporters family signature. LSGGEWQRVRLAAVV |
| Chain | Residue | Details |
| C | LEU127-VAL141 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| F | TYR50 | |
| B | GLN207-LEU228 | |
| B | GLY268-VAL274 | |
| B | GLY325-ARG326 | |
| D | GLY33 | |
| A | HIS139-SER141 | |
| A | GLN207-LEU228 | |
| A | GLY268-VAL274 | |
| A | GLY325-ARG326 | |
| B | MET1-ARG10 | |
| B | GLU82-GLY92 | |
| B | HIS139-SER141 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0000269|PubMed:12475936, ECO:0007744|PDB:1N2Z, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| F | ASP242 | |
| B | GLN11-GLU35 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Important for BtuC binding => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000305|PubMed:12475936 |
| Chain | Residue | Details |
| F | GLU72 | |
| B | ALA297-PRO304 | |
| F | GLU202 | |
| A | SER167-GLN190 | |
| A | ALA250-GLY257 | |
| A | ALA297-PRO304 | |
| B | GLN36-ARG56 | |
| B | GLY108-PRO113 | |
| B | SER167-GLN190 | |
| B | ALA250-GLY257 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=2 |
| Chain | Residue | Details |
| A | LEU57-PHE81 | |
| B | LEU57-PHE81 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 28 |
| Details | TRANSMEM: Helical; Name=3 |
| Chain | Residue | Details |
| A | VAL93-LEU107 | |
| B | VAL93-LEU107 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=4 |
| Chain | Residue | Details |
| A | ASN114-ARG138 | |
| B | ASN114-ARG138 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=5 |
| Chain | Residue | Details |
| A | THR142-PHE166 | |
| B | THR142-PHE166 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 30 |
| Details | TRANSMEM: Helical; Name=6 |
| Chain | Residue | Details |
| A | SER191-SER206 | |
| B | SER191-SER206 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 40 |
| Details | TRANSMEM: Helical; Name=7 |
| Chain | Residue | Details |
| A | TRP229-LEU249 | |
| B | TRP229-LEU249 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 18 |
| Details | TRANSMEM: Helical; Name=8 |
| Chain | Residue | Details |
| A | LEU258-SER267 | |
| B | LEU258-SER267 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=9 |
| Chain | Residue | Details |
| A | LEU275-LEU296 | |
| B | LEU275-LEU296 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 38 |
| Details | TRANSMEM: Helical; Name=10 |
| Chain | Residue | Details |
| A | ILE305-ALA324 | |
| B | ILE305-ALA324 |
