4FGC
Crystal Structure of Active Site Mutant C55A of Nitrile Reductase QueF, Bound to Substrate PreQ0
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | NADPH-dependent 7-cyano-7-deazaguanine reductase | polymer | 165 | 19364.9 | 5 | UniProt (O31678) Pfam (PF14489) | Bacillus subtilis subsp. subtilis | QueF, 7-cyano-7-carbaguanine reductase, NADPH-dependent nitrile oxidoreductase, PreQ(0) reductase |
| 2 | F, J, M, P (B, C, D, E) | 2-AMINO-4-OXO-4,7-DIHYDRO-3H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBONITRILE | non-polymer | 175.1 | 4 | Chemie (PQ0) | |||
| 3 | G, I, K, L, N... (B, C, D, E) | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) | |||
| 4 | H (B) | 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL | non-polymer | 354.4 | 1 | Chemie (PE4) | |||
| 5 | S, T, U, V, W (A, B, C, D, E) | water | water | 18.0 | 205 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 0 - 164 (UniProt: O31678)
| PDB | External Database | Details |
|---|---|---|
| Ala 55 | Cys 56 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 96824.5 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1375.6 | |
| All* | Total formula weight | 98200.1 |
