4FF3

N4 mini-vRNAP transcription initiation complex, 3 min after soaking GTP, ATP and Mn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Virion RNA polymerase	polymer	1118	123193.5	2	UniProt (Q859P9) Pfam (PF21624) Pfam (PF21769) In PDB	Enterobacteria phage N4
2	C, D	Bacteriophage N4 P2 promoter	polymer	36	11022.1	2
3	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
4	A	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
5	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
6	C	GUANOSINE-5'-MONOPHOSPHATE	non-polymer		363.2	1	Chemie (5GP)
7		water	water		18.0	1245	Chemie (HOH)

Sequence modifications

A, B: 1 - 1106 (UniProt: Q859P9)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	268431.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1170.2
All*	Total formula weight	269601.4

*Water molecules are not included.