4FF3

N4 mini-vRNAP transcription initiation complex, 3 min after soaking GTP, ATP and Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-07
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	82.019, 111.652, 276.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.382 - 1.997
R-factor	0.1825
Rwork	0.182
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.863
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.382
High resolution limit [Å]	1.997
Completeness [%]	89.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M TRIS-HCL, 0.21 M DIBASIC AMMONIUM CITRATE, 0.09 M AMMONIUM DIHYDROGEN MONOPHOSPHATE, 25 % (DROP) 15% (RESERVOIR) PEG 3350 , pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K