4F8C

Structure of the Cif:Nedd8 complex - Yersinia pseudotuberculosis Cycle Inhibiting Factor in complex with human Nedd8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Cycle Inhibiting Factor	polymer	275	30809.5	2	UniProt (B1JJZ9) Pfam (PF16374) UniProt (by SIFTS) (A0A0H3B1Q8)	Yersinia pseudotuberculosis
2	B, D (B, D)	NEDD8	polymer	88	10044.6	2	UniProt (Q15843) Pfam (PF00240)	Homo sapiens (human)	Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, Ubiquitin-like protein Nedd8
3	E, F, G, H (A, C)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
4	I, J, K, L (A, B, C, D)	water	water		18.0	318	Chemie (HOH)

Sequence modifications

A, C: 33 - 288 (UniProt: B1JJZ9)

PDB	External Database	Details
Met 14	-	expression tag
Ala 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
Ser 22	-	expression tag
Ser 23	-	expression tag
Gly 24	-	expression tag
Leu 25	-	expression tag
Glu 26	-	expression tag
Val 27	-	expression tag
Leu 28	-	expression tag
Phe 29	-	expression tag
Gln 30	-	expression tag
Gly 31	-	expression tag
Pro 32	-	expression tag
Ala 117	Cys 117	engineered mutation

B, D: 1 - 81 (UniProt: Q15843)

PDB	External Database	Details
Lys 82	-	expression tag
His 83	-	expression tag
His 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	81708.2
Non-Polymers*	Number of molecules	4
	Total formula weight	248.3
All*	Total formula weight	81956.4

*Water molecules are not included.