4F7U

The 6S snRNP assembly intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Small nuclear ribonucleoprotein Sm D1	polymer	119	13310.7	2	UniProt (P62315) Pfam (PF01423) In PDB	Mus musculus (mouse)	Sm-D1, Sm-D autoantigen, snRNP core protein D1
2	B, D	Small nuclear ribonucleoprotein Sm D2	polymer	118	13551.9	2	UniProt (P62317) Pfam (PF01423) In PDB	Mus musculus (mouse)	Sm-D2, snRNP core protein D2
3	E, H	Small nuclear ribonucleoprotein E	polymer	92	10817.6	2	UniProt (P62305) Pfam (PF01423) In PDB	Mus musculus (mouse)	snRNP-E, Sm protein E, Sm-E, SmE
4	F, I	Small nuclear ribonucleoprotein F	polymer	86	9734.2	2	UniProt (P62321) Pfam (PF01423) In PDB	Xenopus laevis (clawed frog common platanna platanna)	snRNP-F, Sm protein F, Sm-F, SmF
5	G, J	Small nuclear ribonucleoprotein G	polymer	76	8508.1	2	UniProt (P62309) Pfam (PF01423) In PDB	Mus musculus (mouse)	snRNP-G, Sm protein G, Sm-G, SmG
6	Q, P	Methylosome subunit pICln	polymer	129	14661.7	2	UniProt (A1ZAW5) Pfam (PF03517) In PDB	Drosophila melanogaster (Fruit fly)	SD10511p
7	H	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
8		water	water		18.0	372	Chemie (HOH)

Sequence modifications

Q, P: 6001 - 6125 (UniProt: A1ZAW5)

PDB	External Database	Details
His 6006	Arg 6	SEE REMARK 999

Q, P: 6126 - 6160 (UniProt: A1ZAW5)

PDB	External Database	Details
Ala 6144	His 144	engineered mutation
His 6161	-	expression tag
His 6162	-	expression tag
His 6163	-	expression tag
His 6164	-	expression tag
His 6165	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	141168.2
Non-Polymers*	Number of molecules	1
	Total formula weight	282.3
All*	Total formula weight	141450.5

*Water molecules are not included.