4F6Z
Mutagenesis of zinc ligand residue Cys221 reveals plasticity in the IMP-1 metallo-b-lactamase active site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Beta-lactamase | polymer | 236 | 26142.7 | 1 | UniProt (Q79MP6) Pfam (PF00753) | Pseudomonas aeruginosa | Beta-lactamase IMP-1, Bla-imp protein, Extended-spectrum B-lactamase, IMP-1 metallo-beta-lactmase, Metallo beta lactamase, Metallo-beta-lactamase, Metallo-beta-lactamase IMP-1, Metallo-beta-lactamase blaIMP-1 |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C (A) | CITRATE ANION | non-polymer | 189.1 | 1 | Chemie (FLC) | |||
| 4 | D (A) | water | water | 18.0 | 151 | Chemie (HOH) |
Sequence modifications
A: 1 - 228 (UniProt: Q79MP6)
| PDB | External Database | Details |
|---|---|---|
| Gly 158 | Cys 176 | engineered mutation |
| Trp 229 | - | expression tag |
| Ser 230 | - | expression tag |
| His 231 | - | expression tag |
| Pro 232 | - | expression tag |
| Gln 233 | - | expression tag |
| Phe 234 | - | expression tag |
| Glu 235 | - | expression tag |
| Lys 236 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 26142.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 254.5 | |
| All* | Total formula weight | 26397.2 |
