4F60

Crystal structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant (T148L, G171Q, A172V, C176F).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Haloalkane dehalogenase	polymer	299	34266.1	1	UniProt (P0A3G2) Pfam (PF00561)	Rhodococcus rhodochrous
2	B (A)	FLUORIDE ION	non-polymer		19.0	1	Chemie (F)
3	C (A)	water	water		18.0	500	Chemie (HOH)

Sequence modifications

A: 1 - 293 (UniProt: P0A3G2)

PDB	External Database	Details
Leu 148	Thr 148	engineered mutation
Gln 171	Gly 171	engineered mutation
Val 172	Ala 172	engineered mutation
Phe 176	Cys 176	engineered mutation
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34266.1
Non-Polymers*	Number of molecules	1
	Total formula weight	19.0
All*	Total formula weight	34285.1

*Water molecules are not included.