4F52

Structure of a Glomulin-RBX1-CUL1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Cullin-1	polymer	282	32470.1	2	UniProt (Q13616) Pfam (PF00888)	Homo sapiens (human)	CUL-1
2	B, D (B, D)	E3 ubiquitin-protein ligase RBX1	polymer	106	12089.7	2	UniProt (P62877) Pfam (PF12678)	Homo sapiens (human)	Protein ZYP, RING finger protein 75, RING-box protein 1, Rbx1, Regulator of cullins 1
3	E, F (E, F)	Glomulin	polymer	596	68425.1	2	UniProt (Q92990) Pfam (PF08568)	Homo sapiens (human)	FK506-binding protein-associated protein, FAP, FKBP-associated protein
4	G, H, I, J, K... (B, D)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A, C: 411 - 690 (UniProt: Q13616)

PDB	External Database	Details
Gly 409	-	expression tag
Ser 410	-	expression tag
Glu 421	Leu 421	engineered mutation
Glu 451	Val 451	engineered mutation
Lys 452	Val 452	engineered mutation
Lys 455	Tyr 455	engineered mutation

B, D: 5 - 108 (UniProt: P62877)

PDB	External Database	Details
Gly 3	-	expression tag
Ser 4	-	expression tag

E, F: 1 - 594 (UniProt: Q92990)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	225969.7
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	226362.2

*Water molecules are not included.