4F2S
DNA Polymerase I Large Fragment complex 4
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, D) | DNA polymerase | polymer | 592 | 67490.3 | 2 | UniProt (Q5KWC1) Pfam (PF22619) Pfam (PF00476) | Geobacillus kaustophilus | |
| 2 | C, E (B, E) | DNA (5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DOC))-3') | polymer | 9 | 2635.8 | 2 | synthetic construct | ||
| 3 | D, F (C, F) | DNA (5'-D(*CP*AP*TP*GP*GP*GP*AP*GP*TP*CP*AP*GP*G)-3') | polymer | 13 | 4056.6 | 2 | synthetic construct | ||
| 4 | G, H (G, H) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
| 5 | I, J (A, D) | CYTIDINE-5'-TRIPHOSPHATE | non-polymer | 483.2 | 2 | Chemie (CTP) | |||
| 6 | K (D) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 7 | L (D) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 8 | M, N, O, P, Q... (A, D, B, C, E...) | water | water | 18.0 | 1323 | Chemie (HOH) |
Sequence modifications
A, D: 285 - 876 (UniProt: Q5KWC1)
| PDB | External Database | Details |
|---|---|---|
| Ala 598 | Asp 600 | engineered mutation |
| Tyr 710 | Phe 712 | engineered mutation |
| His 823 | Arg 825 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 148365.4 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1117.3 | |
| All* | Total formula weight | 150167.3 |
