4F2S
DNA Polymerase I Large Fragment complex 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2011-11-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.180, 108.590, 150.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.190 - 1.651 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.19940 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4f2r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.403 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (dev_1026) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 4.940 | 1.650 |
| Rmerge | 0.109 | 0.079 | 0.509 |
| Number of reflections | 180667 | 7247 | 26285 |
| <I/σ(I)> | 15 | 31.96 | 3.72 |
| Completeness [%] | 98.2 | 99.8 | 89.1 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 47%-49% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MnSO4, 100mM MES , pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
