4F1M

Crystal Structure of the G1179S Roco4 Kinase Domain bound to AppCp from D. discoideum.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Serine/threonine-protein kinase roco4	polymer	287	32646.6	1	UniProt (Q6XHB2) Pfam (PF07714) In PDB	Dictyostelium discoideum (Slime mold)	Ras of complex proteins and C-terminal of roc 4
2	A	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
3	A	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	1	Chemie (BTB)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5		water	water		18.0	96	Chemie (HOH)

Sequence modifications

A: 1019 - 1292 (UniProt: Q6XHB2)

PDB	External Database	Details
Gly 1006	-	expression tag
Ala 1007	-	expression tag
Met 1008	-	expression tag
Gly 1009	-	expression tag
Gly 1010	-	expression tag
Ser 1011	-	expression tag
Glu 1012	-	expression tag
Phe 1013	-	expression tag
Pro 1014	-	expression tag
Lys 1015	-	expression tag
Ser 1016	-	expression tag
Arg 1017	-	expression tag
Leu 1018	-	expression tag
Ser 1179	Gly 1179	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32646.6
Non-Polymers*	Number of molecules	3
	Total formula weight	738.8
All*	Total formula weight	33385.3

*Water molecules are not included.