4EXM

The crystal structure of an engineered phage lysin containing the binding domain of pesticin and the killing domain of T4-lysozyme

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Pesticin, Lysozyme Chimera	polymer	347	39700.0	4	UniProt (Q57159) UniProt (P00720) Pfam (PF21613) Pfam (PF00959)	Yersinia pestis	Endolysin, Lysis protein, Muramidase
2	E, F, G, H (A, B, C, D)	water	water		18.0	75	Chemie (HOH)

Sequence modifications

A, B, C, D: 6 - 172 (UniProt: Q57159)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Tyr 2	-	expression tag
Phe 3	-	expression tag
Gln 4	-	expression tag
Ser 5	-	expression tag

A, B, C, D: 173 - 334 (UniProt: P00720)

PDB	External Database	Details
Arg 182	Gly 13	engineered mutation
Thr 224	Cys 55	engineered mutation
Ala 267	Cys 98	engineered mutation
Ile 307	Arg 138	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	158800.0
All*	Total formula weight	158800.0

*Water molecules are not included.