4EXM

The crystal structure of an engineered phage lysin containing the binding domain of pesticin and the killing domain of T4-lysozyme

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003796	molecular_function	lysozyme activity
A	0009253	biological_process	peptidoglycan catabolic process
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0016998	biological_process	cell wall macromolecule catabolic process
A	0030430	cellular_component	host cell cytoplasm
A	0031640	biological_process	killing of cells of another organism
A	0042742	biological_process	defense response to bacterium
A	0044659	biological_process	viral release from host cell by cytolysis
B	0003796	molecular_function	lysozyme activity
B	0009253	biological_process	peptidoglycan catabolic process
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0016998	biological_process	cell wall macromolecule catabolic process
B	0030430	cellular_component	host cell cytoplasm
B	0031640	biological_process	killing of cells of another organism
B	0042742	biological_process	defense response to bacterium
B	0044659	biological_process	viral release from host cell by cytolysis
C	0003796	molecular_function	lysozyme activity
C	0009253	biological_process	peptidoglycan catabolic process
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0016998	biological_process	cell wall macromolecule catabolic process
C	0030430	cellular_component	host cell cytoplasm
C	0031640	biological_process	killing of cells of another organism
C	0042742	biological_process	defense response to bacterium
C	0044659	biological_process	viral release from host cell by cytolysis
D	0003796	molecular_function	lysozyme activity
D	0009253	biological_process	peptidoglycan catabolic process
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0016998	biological_process	cell wall macromolecule catabolic process
D	0030430	cellular_component	host cell cytoplasm
D	0031640	biological_process	killing of cells of another organism
D	0042742	biological_process	defense response to bacterium
D	0044659	biological_process	viral release from host cell by cytolysis

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:3382407, ECO:0000269|PubMed:7831309, ECO:0000269|PubMed:8266098

Chain	Residue	Details
A	ASP180
B	ASP180
C	ASP180
D	ASP180

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site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:1892846, ECO:0000269|PubMed:3382407, ECO:0000269|PubMed:7831309, ECO:0000269|PubMed:8266098

Chain	Residue	Details
A	LYS189
B	LYS189
C	LYS189
D	LYS189

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site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:8266098

Chain	Residue	Details
A	HIS201
A	VAL273
B	HIS201
B	VAL273
C	HIS201
C	VAL273
D	HIS201
D	VAL273

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site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000303|PubMed:7831309

Chain	Residue	Details
A	ASN286
A	VAL301
B	ASN286
B	VAL301
C	ASN286
C	VAL301
D	ASN286
D	VAL301

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
A	ASP180	proton shuttle (general acid/base)
A	LYS189	covalent catalysis

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site_id	MCSA2
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
B	ASP180	proton shuttle (general acid/base)
B	LYS189	covalent catalysis

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site_id	MCSA3
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
C	ASP180	proton shuttle (general acid/base)
C	LYS189	covalent catalysis

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site_id	MCSA4
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
D	ASP180	proton shuttle (general acid/base)
D	LYS189	covalent catalysis

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