4EUL
Crystal structure of enhanced Green Fluorescent Protein to 1.35A resolution reveals alternative conformations for Glu222
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green fluorescent protein | polymer | 239 | 26956.4 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria (Jellyfish) | |
| 2 | B, C (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | D, E, F, H (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 4 | Chemie (PEG) | |||
| 4 | G (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | I (A) | water | water | 18.0 | 389 | Chemie (HOH) |
Sequence modifications
A: 2 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Val 1 | - | expression tag |
| Leu 64 | Phe 64 | engineered mutation |
| Cro 66 | Ser 65 | chromophore |
| Cro 66 | Tyr 66 | chromophore |
| Cro 66 | Gly 67 | chromophore |
| Leu 231 | His 231 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 26956.4 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 600.7 | |
| All* | Total formula weight | 27557.1 |
