4EUL
Crystal structure of enhanced Green Fluorescent Protein to 1.35A resolution reveals alternative conformations for Glu222
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97988 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.114, 62.246, 69.641 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.420 - 1.350 |
| R-factor | 0.13041 |
| Rwork | 0.128 |
| R-free | 0.16892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y0g |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.989 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.420 |
| High resolution limit [Å] | 1.350 |
| Rmerge | 0.093 |
| Number of reflections | 49389 |
| <I/σ(I)> | 5 |
| Completeness [%] | 99.8 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES pH 6.5, 200 mM calcium acetate, 20% (w/v) PEG8000, 13% PEG200 for cryoprotection, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
