4ETW
Structure of the Enzyme-ACP Substrate Gatekeeper Complex Required for Biotin Synthesis
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Pimelyl-[acyl-carrier protein] methyl ester esterase | polymer | 264 | 29592.1 | 2 | UniProt (Q83PW0) Pfam (PF00561) In PDB | Shigella flexneri | Biotin synthesis protein BioH, Carboxylesterase BioH |
2 | D, B | Acyl carrier protein | polymer | 77 | 8514.3 | 2 | UniProt (Q0T5U2) Pfam (PF00550) In PDB | Shigella flexneri 5 | ACP |
3 | D, B | methyl 7-{[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}-7-oxoheptanoate | non-polymer | 514.5 | 2 | Chemie (ZMK) | |||
4 | water | water | 18.0 | 223 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 257 (UniProt: Q83PW0)
PDB | External Database | Details |
---|---|---|
Ala 82 | Ser 82 | ENGINEERED MUTATION |
Ala 243 | Val 243 | ENGINEERED MUTATION |
Glu 258 | - | EXPRESSION TAG |
His 259 | - | EXPRESSION TAG |
His 260 | - | EXPRESSION TAG |
His 261 | - | EXPRESSION TAG |
His 262 | - | EXPRESSION TAG |
His 263 | - | EXPRESSION TAG |
His 264 | - | EXPRESSION TAG |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 76212.7 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 1029.1 | |
All* | Total formula weight | 77241.8 |