4ETP

C-terminal motor and motor homology domain of Kar3Vik1 fused to a synthetic heterodimeric coiled coil

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Kinesin-like protein KAR3	polymer	403	45178.9	1	UniProt (P17119) Pfam (PF00225) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Nuclear fusion protein
2	B	Spindle pole body-associated protein VIK1	polymer	333	38478.7	1	UniProt (Q12045) Pfam (PF16796) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Vegetative interaction with KAR3 protein 1
3	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A, B	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
6	B	N,N'-ethane-1,2-diylbis(2-iodoacetamide)	non-polymer		396.0	1	Chemie (EBC)
7		water	water		18.0	246	Chemie (HOH)

Sequence modifications

A: 352 - 729 (UniProt: P17119)

PDB	External Database	Details
Gly 327	-	SEE REMARK 999
Ala 328	-	SEE REMARK 999
Ser 329	-	SEE REMARK 999
Lys 330	-	SEE REMARK 999
Ile 331	-	SEE REMARK 999
Ala 332	-	SEE REMARK 999
Ala 333	-	SEE REMARK 999
Leu 334	-	SEE REMARK 999
Lys 335	-	SEE REMARK 999
Glu 336	-	SEE REMARK 999
Lys 337	-	SEE REMARK 999
Ile 338	-	SEE REMARK 999
Ala 339	-	SEE REMARK 999
Ala 340	-	SEE REMARK 999
Leu 341	-	SEE REMARK 999
Lys 342	-	SEE REMARK 999
Glu 343	-	SEE REMARK 999
Lys 344	-	SEE REMARK 999
Ile 345	-	SEE REMARK 999
Ala 346	-	SEE REMARK 999
Ala 347	-	SEE REMARK 999
Leu 348	-	SEE REMARK 999
Lys 349	-	SEE REMARK 999
Glu 350	-	SEE REMARK 999
Lys 351	-	SEE REMARK 999
Leu 391	Cys 391	engineered mutation
Ala 469	Cys 469	engineered mutation
Ala 517	Cys 517	engineered mutation
Val 566	Cys 566	engineered mutation
Ala 655	Cys 655	engineered mutation

B: 341 - 647 (UniProt: Q12045)

PDB	External Database	Details
Gly 315	-	SEE REMARK 999
Ala 316	-	SEE REMARK 999
Ser 317	-	SEE REMARK 999
Glu 318	-	SEE REMARK 999
Ile 319	-	SEE REMARK 999
Ala 320	-	SEE REMARK 999
Ala 321	-	SEE REMARK 999
Leu 322	-	SEE REMARK 999
Glu 323	-	SEE REMARK 999
Lys 324	-	SEE REMARK 999
Glu 325	-	SEE REMARK 999
Ile 326	-	SEE REMARK 999
Ala 327	-	SEE REMARK 999
Ala 328	-	SEE REMARK 999
Leu 329	-	SEE REMARK 999
Glu 330	-	SEE REMARK 999
Lys 331	-	SEE REMARK 999
Glu 332	-	SEE REMARK 999
Ile 333	-	SEE REMARK 999
Ala 334	-	SEE REMARK 999
Ala 335	-	SEE REMARK 999
Leu 336	-	SEE REMARK 999
Glu 337	-	SEE REMARK 999
Lys 338	-	SEE REMARK 999
Glu 339	-	SEE REMARK 999
Ile 340	-	SEE REMARK 999
Cys 355	Glu 355	engineered mutation
Val 377	Cys 377	engineered mutation
Cys 423	Lys 423	engineered mutation
Ala 436	Cys 436	engineered mutation
Ala 536	Cys 536	engineered mutation
Ala 596	Cys 596	engineered mutation
Ala 640	Cys 640	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83657.7
Non-Polymers*	Number of molecules	9
	Total formula weight	1400.0
All*	Total formula weight	85057.7

*Water molecules are not included.