4ETP
C-terminal motor and motor homology domain of Kar3Vik1 fused to a synthetic heterodimeric coiled coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979206 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.060, 94.939, 114.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.970 - 2.300 |
| R-factor | 0.20923 |
| Rwork | 0.207 |
| R-free | 0.24472 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3KAR and 2O0A |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.891 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.051 | 0.601 |
| Number of reflections | 43179 | |
| <I/σ(I)> | 19.7 | 3.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 277 | 16% monomethyl polyethylene glycol 2000, 100 mM Hepes, 50 mM sodium citrate, Batch, pH 7.5, EVAPORATION, temperature 277K |
