4EN1
The 1.62A structure of a FRET-optimized Cerulean Fluorescent Protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Green fluorescent protein | polymer | 266 | 29589.4 | 2 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria (Jellyfish) | |
| 2 | C, D, E, F, G... (A, B) | GLYCEROL | non-polymer | 92.1 | 10 | Chemie (GOL) | |||
| 3 | H, J, P (A, B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
| 4 | I (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 5 | N (B) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 6 | R, S (A, B) | water | water | 18.0 | 350 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Met -18 | - | initiating methionine |
| Arg -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Val -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Met 0 | - | expression tag |
| Val 1 | - | expression tag |
| Leu 64 | Tyr 66 | engineered mutation |
| Ala 72 | Ser 72 | engineered mutation |
| Ala 145 | Tyr 145 | engineered mutation |
| Ile 146 | Asn 146 | engineered mutation |
| His 147 | Ser 147 | engineered mutation |
| Gly 148 | His 148 | engineered mutation |
| Thr 153 | Met 153 | engineered mutation |
| Ala 163 | Val 163 | engineered mutation |
| Gly 166 | Lys 166 | engineered mutation |
| Leu 167 | Ile 167 | engineered mutation |
| Asn 168 | Arg 168 | engineered mutation |
| Cys 169 | His 169 | engineered mutation |
| Lys 206 | Ala 206 | engineered mutation |
| Leu 231 | His 231 | engineered mutation |
| Ser 239 | - | expression tag |
| Gly 240 | - | expression tag |
| Leu 241 | - | expression tag |
| Arg 242 | - | expression tag |
| Ser 243 | - | expression tag |
| Arg 244 | - | expression tag |
| Ala 245 | - | expression tag |
| Gln 246 | - | expression tag |
| Ala 247 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 59178.9 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1394.4 | |
| All* | Total formula weight | 60573.3 |
