4EN1
The 1.62A structure of a FRET-optimized Cerulean Fluorescent Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-30 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.410, 88.726, 94.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.980 - 1.620 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.071 | 0.477 |
| Number of reflections | 79895 | |
| <I/σ(I)> | 14.5 | 2.04 |
| Completeness [%] | 94.5 | 99 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.7 | 298 | mother liquor consisting of 0.08 M sodium acetate trihydrate, 0.16 M ammonium sulfate, 9% (w/v) PEG4000, and 19% (v/v) glycerol. Hanging drops contained 2 uL protein solution and 1 uL mother liquor, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
