4EKV
Streptavidin 8-aa-loop H127C mutein with reversible biotin binding
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Streptavidin | polymer | 159 | 16286.5 | 1 | UniProt (P22629) | Streptomyces avidinii | |
| 2 | B (A) | BIOTIN | non-polymer | 244.3 | 1 | Chemie (BTN) | |||
| 3 | C (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | D (A) | water | water | 18.0 | 70 | Chemie (HOH) |
Sequence modifications
A: 1 - 159 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Asp 114 | Thr 138 | SEE REMARK 999 |
| Ser 115 | Thr 139 | SEE REMARK 999 |
| Ser 116 | Glu 140 | SEE REMARK 999 |
| Asn 117 | Ala 141 | SEE REMARK 999 |
| Gly 118 | Asn 142 | SEE REMARK 999 |
| Ser 119 | Ala 143 | SEE REMARK 999 |
| Asp 120 | Trp 144 | SEE REMARK 999 |
| Gly 121 | Lys 145 | SEE REMARK 999 |
| Cys 127 | His 151 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 16286.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 279.8 | |
| All* | Total formula weight | 16566.3 |
