4EKD

Structure of human regulator of G protein signaling 2 (RGS2) in complex with murine Galpha-q(R183C)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	347	40634.2	1	UniProt (P21279) Pfam (PF00503)	Mus musculus (mouse)	Guanine nucleotide-binding protein alpha-q
2	B (B)	Regulator of G-protein signaling 2	polymer	137	15871.8	1	UniProt (P41220) Pfam (PF00615)	Homo sapiens (human)	RGS2, Cell growth-inhibiting gene 31 protein, G0/G1 switch regulatory protein 8
3	C (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
4	D (A)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	1	Chemie (ALF)
5	E, H (A)	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
6	F, G (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	I (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	J (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
9	K, L (A, B)	water	water		18.0	16	Chemie (HOH)

Sequence modifications

A: 18 - 359 (UniProt: P21279)

PDB	External Database	Details
Gly 13	-	expression tag
Ala 14	-	expression tag
Met 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
Asp 125	Glu 125	engineered mutation
Val 126	Asn 126	engineered mutation
Asp 128	Tyr 128	engineered mutation
Tyr 129	Val 129	engineered mutation
Ala 130	Asp 130	engineered mutation
Cys 183	Arg 183	engineered mutation

B: 72 - 203 (UniProt: P41220)

PDB	External Database	Details
Gly 67	-	expression tag
Glu 68	-	expression tag
Phe 69	-	expression tag
Gly 70	-	expression tag
Ser 71	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56506.0
Non-Polymers*	Number of molecules	8
	Total formula weight	954.5
All*	Total formula weight	57460.5

*Water molecules are not included.