4EGC

Crystal Structure of MBP-fused Human Six1 Bound to Human Eya2 Eya Domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose-binding periplasmic protein, Homeobox protein SIX1 chimera	polymer	559	62503.0	1	UniProt (P0AEX9) UniProt (Q15475) Pfam (PF01547) Pfam (PF16878) Pfam (PF05920) In PDB	Escherichia coli (human)	MBP, MMBP, Maltodextrin-binding protein, Sine oculis homeobox homolog 1
2	B	Eyes absent homolog 2	polymer	294	33026.3	1	UniProt (O00167) Pfam (PF00702) In PDB	Homo sapiens (human)	EYA2
3	C	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5		water	water		18.0	812	Chemie (HOH)

Sequence modifications

A: 1 - 366 (UniProt: P0AEX9)

PDB	External Database	Details
Ala 172	Glu 198	engineered mutation
Ala 173	Asn 199	engineered mutation
Ala 359	Glu 385	engineered mutation
Ala 362	Lys 388	engineered mutation
Ala 363	Asp 389	engineered mutation
Asn 367	-	expression tag
Ala 368	-	expression tag
Ala 369	-	expression tag
Ala 370	-	expression tag

A: 371 - 559 (UniProt: Q15475)
B: 253 - 538 (UniProt: O00167)

PDB	External Database	Details
Gly 245	-	expression tag
Pro 246	-	expression tag
Leu 247	-	expression tag
Gly 248	-	expression tag
Ser 249	-	expression tag
Pro 250	-	expression tag
Glu 251	-	expression tag
Phe 252	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95529.3
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	95895.9

*Water molecules are not included.