4EGC

Crystal Structure of MBP-fused Human Six1 Bound to Human Eya2 Eya Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	85
Detector technology	CCD
Collection date	2011-07-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97912
Spacegroup name	P 21 21 2
Unit cell lengths	123.207, 150.180, 53.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.032 - 1.994
R-factor	0.1861
Rwork	0.184
R-free	0.22410
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.045
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.480	4.020	1.480
Rmerge	0.081	0.031
Number of reflections	145786
<I/σ(I)>	4.6
Completeness [%]	87.6	99.7	39.8
Redundancy	7.1	9	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	291	13.75% PEG8000, 0.01 M magnesium chloride, 0.05 M MES, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K