4EGC
Crystal Structure of MBP-fused Human Six1 Bound to Human Eya2 Eya Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2011-07-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97912 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 123.207, 150.180, 53.921 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.032 - 1.994 |
R-factor | 0.1861 |
Rwork | 0.184 |
R-free | 0.22410 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.045 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.510 |
High resolution limit [Å] | 1.480 | 4.020 | 1.480 |
Rmerge | 0.081 | 0.031 | |
Number of reflections | 145786 | ||
<I/σ(I)> | 4.6 | ||
Completeness [%] | 87.6 | 99.7 | 39.8 |
Redundancy | 7.1 | 9 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 291 | 13.75% PEG8000, 0.01 M magnesium chloride, 0.05 M MES, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |