4EBK

Crystal structure of aminoglycoside 4'-O-adenylyltransferase ANT(4')-IIb, tobramycin-bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Aminoglycoside nucleotidyltransferase	polymer	272	30824.6	2	UniProt (Q83V96) Pfam (PF01909) Pfam (PF18280) UniProt (by SIFTS) (D0E7M2)	Pseudomonas aeruginosa
2	C, W (A, B)	TOBRAMYCIN	non-polymer		467.5	2	Chemie (TOY)
3	D, X (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	AA, BA, CA, DA, E... (B, A)	IODIDE ION	non-polymer		126.9	12	Chemie (IOD)
5	EA, FA, GA, HA, IA... (B, A)	CHLORIDE ION	non-polymer		35.5	17	Chemie (CL)
6	JA, KA (A, B)	water	water		18.0	475	Chemie (HOH)

Sequence modifications

A, B: 2 - 251 (UniProt: Q83V96)

PDB	External Database	Details
Mse -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Arg -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Val 1	-	expression tag
Leu 111	Met 111	SEE REMARK 999
Asp 127	Asn 127	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61649.2
Non-Polymers*	Number of molecules	33
	Total formula weight	3184.7
All*	Total formula weight	64833.9

*Water molecules are not included.